DNA RESEARCH; STUDIES FROM YEREVAN STATE UNIVERSITY, DEPARTMENT OF BIOPHYSICS HAVE PROVIDED NEW DATA ON DNA RESEARCH
Proteomics Weekly
June 30, 2008
Researchers detail in ‘Influence of ionic strength on Hoechst 33258
binding with DNA,’ new data in dna research. "The binding of Hoechst
33258 with DNA at various ionic strengths of solution and different
ligand concentrations has been investigated. Existence of more than
one type of interactions of Hoechst 33258 with DNA has been revealed,
which were very sensitive to the ionic strength," researchers in
Yerevan, Armenia report (see also DNA Research).
"Hoechst 33258 doesn’t show specificity to AT sequences of DNA at
low ionic strength. High affinity binding mode becomes obvious at
high ionic strength," wrote P.O. Vardevanyan and colleagues, Yerevan
State University, Department of Biophysics.
The researchers concluded: "The values of binding constants and
binding site sizes for revealed strong and weak interactions have
been determined."
Vardevanyan and colleagues published their study in the Journal of
Biomolecular Structure and Dynamics (Influence of ionic strength on
Hoechst 33258 binding with DNA. Journal of Biomolecular Structure
and Dynamics, 2008;25(6):641-6).
For additional information, contact P.O. Vardevanyan, Yerevan State
University, Dept. of Biophysics, Yerevan, 375025, Armenia.
Publisher contact information for the Journal of Biomolecular Structure
and Dynamics is: Adenine Press, 2066 Central Avenue, Schenectady,
NY 12304, USA.